Profil Ausstattung
Forschungsprofil
The central role of MEC/MES is to act as a versatile partner for the clinical surgery team to develop and implement modern patient care for our patients. With a focus on visceral and hepatobiliary oncology, our goal is provide the right treatment, at the right time to the individual disease background of each patient. We use a variety of cellular and molecular technologies to identify and investigate mechanisms of biomarkers allowing personalization and optimization of therapy and therapy surveillance. Moreover, by implementing labelfree and labeling-based approaches, alongside cooperation campaigns with experts from electrical engineering, we use this platform to develop next stage functional cancer imaging strategies that support the intro-operative surgical guidance. We focus on human-based disease modeling and standardization plus digitalization of lab procedures and documentation, thus allowing repeatable and open science meanwhile supporting the 3R movement in biomedical research. We believe our research is in line with current science policy and of current technology level.
Expertise:
Expertise:
- Patient-derived cancer stem cell research: primary organoid technology for pancreas, colon, rectum, hepatic and cholangiocarcinoma and its patient-matching, organ-equivalent non-cancer tissue, from resection and biopsy material
- Human induced pluripotent stem cells (hiPSC): generation new hiPSC lines from donor-derived somatic cells, differentiation of hiPSC into hepatic and intestinal lineage
- Biobanking system for body fluids and tissue sample
- Neuro biology: in vitro brain tumor models: stem cell models for transcriptional and DNA methylation subtypes of glioblastoma, low grade glioma models, pediatric brain tumor models featuring MYC-medulloblastomas and glioma, differentiation of hiPSC into neural stem cells and neural crest cells, terminal differentiation into sensory neurons, sphere size quantification
- Genetic engineering: gene/microRNA expression modulation via interference or overexpression using transient and stable transformation of target matrix, single nucleotide editing (mono and bi allelic)
- Generation of therapy resistance in vitro models using stress or biomarker-driven approaches
- Co-culture assays tumor cells with components of the tumor microenvironment
- Biomarker analytics: qPCR, protein assays such as Western blot, FACS, ELISA, CRISPR-Cas13 diagnostics, immune histo and immune fluorescence cytochemistry
- Functional assays: quantification of cellular survival, growth, apoptosis, necrosis, ferroptosis, motility, invasiveness, uptake of intervention/reporter dye, stemness, clonogenicity, chemo- and radio therapy resistance,
- Cell metabolomics (glutaminolysis and ATP consumption)
- Electronic lab notebook management system (ElabFTW)
- Systematic review of scientific literature and meta-analysis
Geräte/Ausrüstungen
Personen Projekte Kooperationen
Personenliste
Projektliste
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Kooperationsliste
- Albert-Ludwigs-Universität Freiburg (Br.), imtek
- Albert-Ludwigs-Universität Freiburg, Klinik für Innere Medizin II
- Albert-Ludwigs-Universität Freiburg, Klinik für Neurochirurgie, Tumorgenetik
- Berlin Institute of Health (BIH)
- Deutsches Krebsforschungszentrum (DKFZ), Heidelberg, Cancer Progression and Metastasis
- Dr. Margarete Fischer-Bosch-Institut für Klinische Pharmakologie
- Hebrew University, Jerusalem, Klinik für Neurologie
- Heinrich-Heine Universität Düsseldorf
- Heinrich-Heine Universität Düsseldorf, Klinik für Neurochirurgie, Abteilung Informatik
- Heinrich-Heine Universität Düsseldorf, Klinik für Neurologie
- Heinrich-Heine Universität Düsseldorf, Nanoporöse und Nanoskalierte Materialien
- Helmholtz Zentrum München, Campus Neuherberg, Therapeutic Antibodies
- Institute of Oncology Research, Bellinzona, Experimental Therapeutics
- Johns Hopkins University, Baltimore, USA, Abteilung Pathologie, Neuropathologie
- Leibniz-Institut für umweltmedizinische Forschung
- Mass General Cancer Center, Boston, Neuro-Oncology
- Max-Delbrück-Centrum für Molekulare Medizin in der Helmholtz-Gemeinschaft, Translationale Onkologie solider Tumore
- Medizinische Hochschule Hannover, Institut Experimentelle Hämatologie
- Otto von Guericke Universität Magdeburg, Institut für Molekulare und Klinische Immunologie (IMKI)
- Otto von Guericke Universität Magdeburg, Campus Stimulate , Elektrotechnik
- Otto von Guericke Universität Magdeburg, Campus Stimulate , Maschinenbau
- Otto von Guericke Universität Magdeburg, Klinik für Hämatologie und Onkologie
- Otto von Guericke Universität Magdeburg, Klinik für Radiologie und Nuklearmedizin
- Otto von Guericke Universität Magdeburg, Universitätskinderklinik
- Otto von Guericke Universität Magdeburg, Universitätsklinik für Gastroenterologie, Hepatologie und Infektiologie
- Otto von Guericke Universität Magdeburg; Institut Pathologie, Exp. Pathologie
- Otto von Guericke Universität, Klinik für Neurochirurgie
- Queen s University Belfast, Northern Ireland, School of Pharmacy
- Ruhr-Universität Bochum, Abteilung Anatomie und Molekulare Embryologie
- Samsung Medical Center, Neurosurgery, Seoul
- TU Dresden, Klinik und Poliklinik für Viszeral-, Thorax- und Gefäßchirurgie
- TU Dresden, Leibniz-Institut für Polymerforschung Dresden e.V. (IPF)
- Technische Univeristät München, Elektrotechnik und Informationstechnik
- Tel Aviv Universität, Cell Research and Immunology
- Tiantan Hospital Beijing
- University of Edinburgh, Neurology and Translational Neuroscience
- University of Groningen, Medical Oncology
- Universität zu Köln, Klinik I für Innere Medizin
- Universität zu Köln, Klinik und Poliklinik für Allgemein-, Viszeral-, Tumor- und Transplantationschirurgie
- Universität zu Köln; Klinik für Neurochirurgie
Publikationen
Publikationsliste
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