HAPPAN: Haplotype und pan-genomische Erschließung der multiparentalen Wildgerste Population HEB-25 zur vollständigen Nutzbarmachung der Ressource für die Züchtung neuer Gerstesorten mit verbesserter Anpassung an sich verändernden Umweltbedingungen.

The HAPPAN project aims to establish a genome reference sequence of a wild barley accession of the HEB-25 population. In addition, full sequencing of all 25 wild barley parents of HEB-25 will be achieved. By comparing domesticated and wild barley we expect to identify locations of large structural variations and to define the wild-barley pan-genome. Subsequently the pan genome data will be used to decode the entire population of 1,420 lines of the wild barley nested association mapping (NAM) population HEB-25. This will allow to detect large sequence variations and define an ultra-high density genome-wide set of DNA markers, including novel variations and genes that are untapped in domesticated barley lines.
Integration of recently published GBS marker data from ~20.000 accessions (from IPK Genbank) will facilitate to link our resource to the global barley collection. Furthermore, the genome-wide data of all 25 wild accessions will allow to define a HAP100k core SNP dataset. Here, we define the next panel design that is applicable to genotype large collections, with improved representation of the gene set and comprising a large amount of novel alleles.