Chloroplast RNA polymerase activity during acclimation

This project aims to quantify transcriptional alterations that occur in chloroplasts during acclimation to changes in light or temperature. It will apply chloroplast nascent transcript sequencing strategies (GRO-seq and 5’-GRO-seq) in order to globally assess transcriptional contributions to changes in chloroplast gene expression during acclimation. By combining high-resolution quantitative transcriptional analyses with a reverse genetic approach we will determine distinct contributions of individual chloroplast RNA polymerases to transcriptional alterations.