Purification and Characterization of Vaccinia virus with special emphasis on MVA-BN®

Development of an affinity chromatography purification of cell culture derived Vaccinia Virus (VV) after an initial host cell homogenization and clearance centrifugation. The affinity chromatography is based on the interaction between the VV surface protein A27L and heparin, which is currently further characterized by surface plasmon resonance technology. In addition, heparin like molecules are investigated. Moreover, classical ion exchange membrane chromatography and cellufine sulfate with heparin derivatized membrane chromatography are characterized including the removal rate of contaminating host cell proteins and DNA.