Stellate cells (PSCs) are important for the development of pancreatic diseases. Especially in pancreatic cancer, their function of inducing the desmoplastic reaction is essential. Once activated, they are able to produce and deposit large amounts of collagen that causes hypoxic conditions supporting tumor growth. Somatic mutations in pancreatic stellate cells may play a critical role in tumor development. Therefore, in this study, we aim to identify and characterize genomic alterations in this specific cell type.