GRK 2408: Maladaptive Prozesse an physiologischen Grenzflächen bei chronischen Erkrankungen; Teilprojekt 6: Normoxic HIF stabilization at the vascular barrier in atherosclerosis

Hypothesis: We hypothesize that proatherogenic micromilieu factors induce normoxic HIF stabilization, leading to molecular fixation of atherogenic maladaptation and loss of EndoC barrier function.

Aims

1. Systematic analysis of the effect of atherosclerosis-associated micromilieu factors on HIF-1a stabilization under normoxic conditions using a HCS with subsequent imaging-independent verification
2. Functional characterization of HIF stabilizing agents with regard to barrier function and modulation of the EndoC secretome

Normoxic HIF stabilization at the vascular barrier in atherosclerosis

Hypothesis: We hypothesize that proatherogenic micromilieu factors induce normoxic HIF stabilization, leading to molecular fixation of atherogenic maladaptation and loss of EndoC barrier function.

Aims 

1. Systematic analysis of the effect of atherosclerosis-associated micromilieu factors on HIF-1a stabilization under normoxic conditions using a HCS with subsequent imaging-independent verification
2. Functional characterization of HIF stabilizing agents with regard to barrier function and modulation of the EndoC secretome