TP 05 Charakterisierung der mikrobiellen Dehalogenierung mittels substanzspezifischer Isotopenanalyse

Follow-up Project of MICROISOTOPE- FOR1530
In Phase 2 similar aims will be pursued:

Characterization of the microbial reductive dehalogenation reaction via stable isotope techniques.

Although organohalide respiration has been investigated over the last few decades, it is still unclear
which reaction mechanisms are involved. Although similar pathways and enzymes are thought to be
involved in different organohalide respireres, our recent research comparing the reductive
dechlorination of chlorinated ethenes in a variety of microbial strains showed a high variability in
apparent kinetic carbon isotope fractionation. The variability of apparent kinetic isotope effects gives
rise to the hypothesis that pathways and mechanisms of dehalogenation reactions are slightly
different and a systematic study comparing the enzymatic inventory and fractionation factors is
needed for a better understanding of the dehalogenation process.