« Projekte
Sie verwenden einen sehr veralteten Browser und können Funktionen dieser Seite nur sehr eingeschränkt nutzen. Bitte aktualisieren Sie Ihren Browser. http://www.browser-update.org/de/update.html
In vitro endothelialization of small caliber vascular prostheses made of bacterial nanocellulose depending on coating with albumin, fibronectin or heparin.
Projektleiter:
Projektbearbeiter:
Dr. rer. nat. Jörn Hülsmann, Dr. Priya Veluswamy, Jan Riedel, Prof. Dr. med. Jens Wippermann, Dr. med. Maximilian Scherner
Finanzierung:
Deutsche Forschungsgemeinschaft (DFG) ;
In vitro endothelialization of small caliber vascular prostheses made of bacterial nanocellulose depending on coating with albumin, fibronectin or heparin.

Coronary artery bypass grafting is the most frequent heart operation in western society. Many patients do not have enough autologous graft material. Alternatives, such as synthetic grafts consisting of plastic fibre, did not show reasonable results. These poor results were mainly attributed to mechanical instability and high thrombogenicity. We managed to produce innovative vascular grafts consisting of bacterial nanocellulose (BNC). These grafts have mechanical properties comparable to autologous vessels. First experiments showed a high occlusion rate, mainly due to lack of endothelialization followed by thrombosis. Reaching endothelialization of vascular grafts by fishing for endothelial progenitor cells circulating in the blood is considered the holy grail of vascular tissue engineering. Recent studies report increased endothelialization of BNC after specific coating. In our own previous work we successfully coated BNC with albumin, fibronectin and heparin. In a preliminary experiment we showed increased expansion of vascular endothelial cells, isolated from veins, on the coated BNC grafts. The aim of this study is to evaluate the coating’s impact on endothelialization under physiologic conditions. Therefore, we use a bioreactor that is already established in our laboratory. In this bioreactor, coated and uncoated tubes are seeded with human and ovine vascular endothelial cells or endothelial progenitor cells and perfused with cell culture media under in-vivo conditions regarding flow, pressure and temperature. After cultivation for three days, the endothelial cells expanded on the graft surface are quantified and functionally characterized. We expect that the coatings lead to increased endothelialization with endothelial progenitor cells. The results of this study are substantial for future strategies of developing alternative graft materials and will be the basis for following in-vivo animal experiments.
Kontakt

weitere Projekte

Die Daten werden geladen ...