Detektion von Jasmonaten
Jasmonic acid (JA) and its derivatives collectively known as jasmonates belong to a family of lipid-derived signaling molecules that affects many aspects of plant life, including defense against pathogens and symbiontic interactions with mycorrhizal fungi. Most functions of jasmonates have been identified through analysis of mutants that are JA-insensitive or affected in the biosynthesis of JA. However, to date, there is no technique available which allows direct detection of JA in a tissue- and cell-specific manner. To broaden our knowledge on the mechanism of JA biosynthesis and perception in response to pathogens and symbionts, a detection system for JA at cell and tissue specific level will be developed by using two different, but complementing approaches: (i) the immunological detection of JA in situ and (ii) the construction of a synthetic promoter.
Jasmonsäure, Mykorrhiza, Zellbiologie
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